Genetic diversity in Jujube germplasm (Ziziphus jujuba Mill.) based on morphological and pomological traits in Isfahan province, Iran


1 Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Isfahan, Iran.

2 Chaharmahal Bakhtyari Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shahrkord, Iran.


Identifying and selecting superior genotypes in native germplasm is one method for breeding fruit trees. Five different ecotypes of Jujube (Ziziphus jujuba Mill.) were collected from different regions of Isfahan province, Iran, for evaluation of their morphological and pomological traits during 2011-13. Results showed that quantitative traits were more significant within ecotypes. ‘‘Najafabad’ ecotype had the highest dimensions of leaves (48×28 mm), fruit weight (2.1 g), and stone weight (0.35g). The largest fruit width (17mm) and peduncle length (13mm) was observed in ‘Ardestan’ ecotype, whereas the largest fruit length (22mm) was observed in ‘Dehaghan’ ecotype. According to the results, the smallest size and weight of fruit, stone weight, and the longest and highest number of annual thorns in shoots were measured in ‘Kouhpayeh’ ecotype. Results showed significant negative and positive correlations between some traits. According to the cluster analysis, ecotypes with desirable traits of fruit were placed in separate clusters from other ecotypes. ‘Najafabad’ ecotype, followed by ‘Ardestan’ and ‘Dehaghan’ ecotypes, can be recommended as promising ecotypes for establishing Jujube orchards and use in Jujube breeding programs in Iran.


Awasthi, O. P., and T. A. More. 2009. Genetic diversity and status of Ziziphus in India. Acta Hort. 840: 33-40.

Bal, J. S. 1992. Identification of Ber (Ziziphus mauritiana) cultivars through vegetative and fruit characters. Hort. Sci. 317: 245-253.

Gao, L., G. F. Zhou, and G. N. Shen. 2003. New jujube varieties and their cultural techniques. China Fruits 2: 38-40.

Gao, W. H., X. G. Li, and C. Z. Wang. 2009. Variation in Morphology of Jujube ‘Muzao’ (Ziziphus jujube Mill.) in the Loess Plateau of China. Acta Hort. 840: 197-202.

Ghosh, S. N., and B. Mathew. 2002. Performance of nine ber (Ziziphus mauritiana Lamk) cultivars on topworking in the semi-arid region of West Bengal. J. App. Hortic. 4: 49-51.

Ghous, K., S. Malekzadeh, M. H. Rashed Mohasel, M. R. Akbari, and S. H. Razavi. 2014. Grouping Jujubes of Iran Based on Quantitative Characteristics and ISSR and RAPD Markers. SPIJ. 30: 173-190.

Grygorieva, O., V. Abrahamova, M. Karnatovska, R. Bleha, and J. Brindza. 2014. Morphological characteristics offruits, drupes and seeds in genotypes of Ziziphus jujuba Mill. Potravinarstvo 8: 306-314.

Khakdaman, H., A. Pourmeidani, and S. M. Adnani. 2007. Study of genetic variation in Iranian jujube (Zyziphus jujuba Mill.) ecotypes. Iran. J. Rangelands For. Plant Breed. Genet. Res. 14: 202-214.

Kohandel, A., M. Pouladian, and A. Yadollahi. 2011. Method of reproduction and breeding Ziziphus jujube Mill. In Proceedings of the first national conference on Barberry and Jujube. Southern Khorasan Province. Birjand, Iran.

Kundi, A. H. K., and F. K. Wazir. 1980. Morphological characteristics, yield and yield components of different cultivars of ber (Ziziphus jujuba Mill.). Sarhad. J. Agric. 5: 53-57.

Li, J. W., S. D. Ding, and X. L. Ding. 2005. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochem. 40: 3607-3613.

Liu, M. 2003. Genetic diversity of Chinese Jujube (Ziziphus jujuba Mill.). Acta Hort. 623: 351-355.

Liu, M. J. 2006. Chinese Jujube: Botany and Horticulture. Hortic. Rev. 32: 229-299.

Liu, P., M. J. Liu, Z. H. Zhao, X. Y. Liu, L. Yang, and Y. L. Wu. 2009. Agronomic Diversity of Sour Jujube (Ziziphus acido jujuba in China. Acta Hort. 840: 203-208.


Liu, Z. G., L. Peng, J. Xiao, Z. Yuan, P. Liu, J. Zhao, and M. J. Liu. 2013. Evaluation of table cultivars of Chinese jujube (Ziziphus jujuba Mill.) in Gravel Gobi of Southern Xinjiang. Acta Hort. 993: 167-172.

Mukhtar, H. M., S. H. Ansari, M. Ali, and T. Naved. 2004. New compounds from Ziziphus vulgaris. Pharm. Biol. 42: 508-511.

Obeed, R. S., A. L. Harhash, and A. L. Abdel Mawgood. 2008. Fruit properties and genetic diversity of five ber (Ziziphus mauritiana Lam.) cultivars. Pak. J. Biol. Sci. 11: 888-893.

Radicevic, S., S. Maric, R. Cerovic, and M. Dordevic. 2013. Assessment of self (in) compatibility in some sweet cherry (Prunu savium L.) genotypes. Genetika 45: 939-952.

Saha, N. N. 1997. Conservation and utilization of fruit plant genetic resources: Bangladesh perspective. In: Hossain, M. G., Aurora, R. K. and Mathur, P. N. (eds.). Dhaka, Bangladesh: BARC-IPGRI.

Saran, P. L. 2005. Studies on genetic divergence in ber (Ziziphus mauritiana Lamk.) germplasm. PhD. thesis, CCSHaryana Agricultural University, Hisar, India.

Shahhoseini, R., A. Babaei, M. Kazemi, and R. Omidbaigi. 2012. A study on genetic variation in Iranian Jujube (Zizyphus jujuba Mill.) genotypes using molecular AFLP marker. Iran. J. Rangelands For. Plant Breed. Genet. Res. 20: 55-68.

Sivakov, L., D. Georgiev, B. Ristevski, andZ. Mitresk. 1988. Pomological and technological characteristics of Chinese jujube (Zyziphus jujuba) in Macedonia. Jugoslovensko Vocarstvo 22: 387-392.

Soltani, M., M. Shamszade, and G. Bisheie. 2011. Review of phenological stages in Ziziphus medicinal plant. In Proceedings of the first national conference on Barberry and Jujube. Southern Khorasan province. Birjand, Iran.

Velkoska Markovska, L., and B. PetanovskaIlievska. 2013. Optimization and development of a spe-hplc-dad method for the determination of atrazine, malathion, fenitrothion, and parathion pesticide residues in apple juice. Maced. J. Chem. Chem. En. 32: 299-308.

Zhao, J., S. P. Li, F. Q. Yang, P. Li, and Y. T. Wang. 2006. Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction. J. Chromatograph. 1108: 188-194.